Fig. 2

Melatonin reduced NaIO₃-induced apoptosis in ARPE-19 cells. After combination treatment with NaIO₃ and melatonin, an Annexin-V/PI assay and MitoPotential kit were used to detect A cell apoptosis. The Y-axis presents the cell viability (%) of normal healthy cells (LL), early and apoptotic cells (LR/UR) and necrotic cells (UL), and B the mitochondrial membrane potential, (upper left): percentage of dead cells with intact mitochondrial membrane; (lower left): percentage of live cells with depolarized mitochondrial membranes. C Cell lysates were analyzed and cleaved caspase-9, cleaved caspase-3, cleaved PARP and cytochrome C was detected by using western blotting (β-actin was used as the internal control). All of the data are presented as the mean ± SEM of three independent experiments. **, P < 0.01 compared with control group, and #, P < 0.05 compared with the NaIO₃ treatment group