Fig. 5

Knockdown of Dot1L alleviates cardiac fibrosis and improves cardiac function during MI. Lentivirus carrying Dot1L shRNA (shDot1L) or Control shRNA (shCtrl) was injected into myocardium of mice undergoing LAD ligation for 14 days to specially knock down the cardiac Dot1L. A The expressions of Dot1L, CTGF, FN, Col 1, and MMP9 in MI mice were analyzed by western blot. B The level of H3K79me3 was analyzed by western blot. C Representative images of immunohistochemistry staining of Dot1L and Col 3 of cross sections of whole heart from MI mice. D Cardiac functions were measured by echocardiography and EF%, FS% were also statistically analyzed. E Representative images of immunofluorescence double staining of Dot1L and MMP9 of cross sections of whole heart. All data are represented as means ± SD. ANOVA, ^***p < 0.001 versus Sham group, ^##p < 0.01, ^###p < 0.001 versus MI treated with shCtrl, n = 6/per group