Fig. 4

Inhibiting Dot1L prevents cardiac fibrosis and improves cardiac function during MI. EPZ5676 at a dose of 20 mg/kg/day was administered to mice undergoing LAD ligation by intraperitoneal injection for 14 days. A The echocardiographic parameters at the 14th day post-MI were statistically analyzed and shown in the graphs, including EF%, FS%, LVIDs and LVIDd. B Masson’s trichrome staining of cross sections of whole heart and Scar size were analyzed. C Western blot was performed to analyze the expression of CTGF, Col 1, MMP9 and FN. D Representative images of immunohistochemistry staining of Col 3. E Representative images of immunofluorescence staining of MMP9. F Representative images of H&E staining at magnification of 50 X and 200 X respectively. Data are represented as means ± SD. ANOVA, ^**p < 0.01, ^***p < 0.001 versus Sham group, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 versus MI, n = 5–8/per group