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Fig. 4 | Cell & Bioscience

Fig. 4

From: Motoneurons innervation determines the distinct gene expressions in multinucleated myofibers

Fig. 4

Innervation determines the different expression between synaptic and non-synaptic region. A Scatter plots of log 10 (FPKM) obtained from innervated samples (SR and NSR) and denervated samples (DSR and DNSR). Red, up-regulated genes in SR or DSR. Green, up-regulated genes in NSR or DNSR. Note that the scatter plot of DNSR vs DSR samples is narrower than that of NSR vs SR samples, indicating less differences in gene expression between the two regions after denervation. FPKM ≥ 1, FC > 1.3, q < 0.001. B Histogram of DEGs number in SR and NSR before and after denervation in A. C Scatter plot shows the trend of alteration of DEGs between SR and NSR after denervation. Black, DEGs between SR and NSR in the sham group. Red, corresponding genes in the sham group after denervation. Note that difference in DEGs in the sham group was reduced after denervation. D GO analysis of genes that reduced their difference after denervation in C. Note that enriched items were opposite in SR and NSR in normal conditions with innervation in Fig. 1F. (I). genes that upregulated in SR (vs. NSR) while lost their difference after denervation. (II). genes that upregulated in NSR (vs. SR) while lost their difference after denervation. E Heat-map of RNA-seq of DEGs in SR and NSR in sham or denervated samples. Hierarchical cluster analysis of these DEGs uncovered the unique changes in pathways across four categories (I-IV, listed on the right). F Real-time PCR analysis of representative synaptic gene expression in SR and NSR after denervation. Three repeats, mean ± SEM, t-test, *p < 0.05

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