Fig. 5
Torin 1 reduced tau phosphorylation and accumulation in human PS1 F105C mutant neurons. A Representative western blotting of tau, P-tau, and α-Tubulin in PS1+/+, PS1+/F105C, and PS1F105C/F105C neurons. B Tau and C P-tau/tau were quantified by western blotting analysis (n = 3). Anti-tau antibody can detect all six isoforms of tau. Data are represented as mean ± SEM. *P < 0.05, and ***P < 0.005. D Tau immunofluorescence staining of neurons. The average intensity of tau in the soma was quantified (n ≥ 50). Neurons were treated with 1 µM Torin 1 or DMSO (vehicle control) for two days. E Tau immunofluorescence staining of neurons with Torin 1 or DMSO treatment. The average intensity of tau in the soma was quantified (n ≥ 50). Data are represented as mean ± SEM. ***P < 0.005 was considered significantly different. F Representative western blotting in PS1+/+, PS1+/F105C, and PS1F105C/F105C neurons. G P-Akt/T-Akt, H P-p70S6K/T-p70S6K, I Tau, and J P-Tau/Tau were quantified by western blotting analysis (n = 3). Data are represented as mean ± SEM. ***P < 0.005 was considered significantly different