Fig. 3
PS1 F105C mutation caused insensitive mTORC1 to starvation in human neurons. A Representative western blotting of p-mTOR, T-mTOR, P-p70S6k, T-p70S6k, P-Akt, T-Akt, and α-Tubulin in PS1+/+, PS1+/F105C, and PS1F105C/F105C neurons after EBSS starvation for 4 h. B p-mTOR/T-mTOR, C P-p70S6k/T-p70S6k, and D P-Akt/T-Akt were quantified by western blotting analysis (n = 3). Data are represented as mean ± SEM. ***P < 0.005 was considered significantly different. E, F Immunofluorescence staining and image J analysis for localization of mTOR and LAMP1 after EBSS starvation in PS1+/+, PS1+/F105C, and PS1F105C/F105C neurons. Co-localized dots were counted (n ≥ 30). Data are represented as mean ± SEM. ***P < 0.005 was considered significantly different