Fig. 2
PS1 F105C mutation enhanced extracellular Aβ levels in human neurons. A The workflow for the neuronal differentiation of iPSCs. B Nestin and SOX2 immunostaining of NSCs from iPSCs and the percentage of Nestin and SOX2 positive cells (n = 3). C TUJ1 immunostaining of neurons from iPSCs and the percentage of TUJ1 positive cells (n = 3). D Representative western blotting of PS1-CTF, PS1-NTF, full-length APP, and α-Tubulin in PS1+/+, PS1+/F105C, and PS1F105C/F105C neurons. Mutation dependent changes in APP in PS1 F105C neurons (n = 3). Data are represented as mean ± SEM. **P < 0.01 and ***P < 0.005 were considered significantly different. ELISA quantification of Aβ40 E and Aβ42 F secreted from neurons (n = 4). Data are represented as mean ± SEM. **P < 0.01 and ***P < 0.005 were considered significantly different. G Mutation dependent changes in Aβ42:40 ratios in PS1 F105C neurons. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005 were considered significantly different