Fig. 2
From: Unraveled roles of Cav1.2 in proliferation and stemness of ameloblastoma
Ca2+ influx was mediated by L-type VGCC in AM cells. A Representative images of Ca2+ intensity in AM cells treated with the DMSO, Bay-k8644 (10 nM), or VPM (10 µM). The Ca2+ transients are indicated with Fluo-4 AM (green), and the depolarization was induced by K-gluconate (50 mM) solutions. Calcium imaging was obtained at 1 s intervals for a total duration of 4 min. B Fmax/F0 (Fmax: Maximum fluorescence intensity upon stimulation) ratios in the Bay-k8644 treatment group was significantly higher than those in the DMSO or VPM treatment group (N = 10 per group, biological replication). C Fluo-4 imaging of the Ca2+ response to K-gluconate in the presence of DMSO, Bay-k8644, or VPM at each time point. Note that the horizontal bar indicates the time of K-gluconate addition. The raw data are expressed as F/F0 (F: fluorescence intensity; F0: mean fluorescence intensity before stimulation). D Immunocytochemistry of the proliferation marker Ki67 in primary AM cells with the presence of DMSO, Bay-k8644, or VPM. White rectangle indicates the high magnification images of Ki67 positive cells. Nuclear was counterstained with TO-PRO-3 (TP3). E Percentage of Ki67 positive cells were quantified from immunostained images (N = 5 per group, biological replication). The Ki67 positive cell percentage in Bay-k8644 treatment group was dramatically increased compared to that in the DMSO or VPM treatment group. Scale bar: 50 μm. *p < 0.05, **p < 0.01