Fig. 6

TRIM22 inhibits DENV and YFV infection in vitro. a, b A549 cells were transfected with TRIM22 expression plasmid or vector control plasmid, 12 h later, the cells were subsequently infected with DENV or YFV at MOI = 0.01. DENV (a) and YFV (b) in A549 cells lysates were measured by qRT-PCR at 24 hpi. c, d Western blot analysis of lysates from HEK293T cells co-transfected with pHis-DENV NS1 and NS3 (c) or pHis-YFV NS1 and NS3 (d), and pM01-TRIM22 or vector control. qRT-PCR data (a, b) are means ± SEM from three independent experiments. **P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t test