Fig. 5From: TRIM22 suppresses Zika virus replication by targeting NS1 and NS3 for proteasomal degradationThe SPRY domain and Ring domain of TRIM22 are required to interact and degrade with NS1 or NS3 protein, respectively. a Schematic diagram of the full length and four truncated forms of TRIM22. b Expression of the four TRIM22 truncations were determined by western blotting. c Whole-cell lysis of HEK293T cells co-transfected with pHis-NS1(upper) or NS3 (lower) and the indicated four truncated forms of TRIM22 were collected for IP with His tag antibody, followed by western blot analysis. d Western blot analysis of lysates from HEK293T cells co-transfected with pHis-NS1 (upper) or pHis-NS3 (lower) and pHA-TRIM22, pHA-TRIM22-Ring-deletion or vector controlBack to article page