Fig. 4

TRIM22 degrade NS1 and NS3 protein by proteasomal degradation. a Western blot analysis of lysates from HEK293T cells co-transfected with pHis-NS1 (left) or pHis-NS3 (right) and pM01-TRIM22 or vector control. b Western blot analysis of the effect of TRIM22 on degradation of NS1 (left) and NS3 (right) proteins. HEK293T cells were transfected with the pHis-NS1 or pHis-NS3 plasmids and an increasing amount of TRIM22 plasmids (0 ng, 250 ng, 500 ng or 1000 ng, wedges) for 24 h, cells were collected and lysates were probed as indicated. c Western blot analysis of lysates from HEK293T cells co-transfected with pM01-TRIM22 and pHis-NS1 (upper) or pHis-NS3 (lower). After 18 h, cells were treated with 10 μM MG132 for 6 h, as indicated. d Western blot analysis of lysates IP from HEK293T cells co-transfected with pHA-ubiquitin, pM01-TRIM22 and pHis-NS1 (left) or pHis-NS3 (right) plasmids and treated with MG132, as indicated