Fig. 3

TRIM22 interacts with NS1 and NS3 protein. a Measurement of binding, entry and replicon efficiency in TRIM22-overexpressing, and TRIM22^−/− A549 cells. b Interactions between TRIM22 and ZIKV nonstructural proteins were screened by a BiLC-based method. Each column represents relative luminescence units from HEK293T cells expressing of individual pair of GlucC-ZIKV and GlucN-TRIM22 compared to controls, detected with Microplate System at 24 h post transfection. c Whole-cell lysis of HEK293T cells co-transfected with pHA-TRIM22 and pHis-NS1 (left) or pHis-NS3 (right) expressing plasmids were collected for IP with indicated antibody, followed by western blot detection. Binding, entry and replicon assays data (a) are means ± SEM from three independent experiments. **P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t test