Fig. 2From: TRIM22 suppresses Zika virus replication by targeting NS1 and NS3 for proteasomal degradationTRIM22 inhibits ZIKV infection in vitro. a–c TRIM22 was over expressed in A549 cells, the cells were subsequently infected with ZIKV at MOI = 0.1, 12 h post transfection, ZIKV load in cells lysates (a) and culture supernatants (b) were measured by qRT-PCR or plaque assay (c). d Western blot analysis of TRIM22 overexpressed A549 cells which were infected with ZIKV. e, f Wild type and TRIM22 gene knockout A549 cells were infected with ZIKV at MOI = 0.01. qRT-PCR was performed to detect the ZIKV RNA in cell lysates (e) and culture supernatant (f). Plaque assay and qRT-PCR data (a–c, e and f) are means ± SEM from three independent experiments. **P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t testBack to article page