Fig. 9
Long-term effects of perinatal intermittent hypoxia on the functional organization of adult mouse cerebellum. A Analysis of the Gluδ2-labeled parallel fiber area (left), and of the Vglut2-labeled climbing fiber area (middle) in the molecular layer of control (N) and hypoxic (IH) mice at P60. Measurements were done from confocal images as illustrated on the right. On Gluδ2 images, parallel fibers appear in green and calbindin-labeled Purkinje dendrites in red. On Vglut2 images, climbing fibers appear in red and calbindin-labeled Purkinje dendrites in green. The white labeling indicates the connection points between fibers and Purkinje dendrites. B Analysis of the Vglut2-labeled mossy fiber area in the granule cell layer (left) of control (N) and hypoxic (IH) mice at P60. Measurements were done from confocal images as illustrated on the right. Red labeling represents mossy fiber rosettes in the granule layer delimited by calbindin-labeled Purkinje cell bodies in green. C Measurement of the length (left) and the volume (right) of myelin binding protein (MBP)-labeled fibers in the granule cell layer in control (N) and hypoxic (IH) mice at P60. Measurements were done from confocal images as illustrated on the right. MBP-labeling (green) indicates that myelin sheaths are mainly located in the granule layer identified by a high DAPI-labeled nucleus density. Each box-plot represents the analysis of 2000 Purkinje cells from four different animals (Calbindin), 750 Purkinje cells from three different animals (Gluδ2), 1230 Purkinje cells from four different animals (Vglut2) and 120 confocal stack images of 10-µm thickness and 370-µm.2 surface from four different animals (MBP). The total number of animals in each experimental group is indicated under the boxplots and represented by diamond shapes, while the transparent dots indicate individual data points. Exact p-values are indicated above the plot. IH: intermittent hypoxia condition; N: normoxia condition; P60: postnatal day 60