Fig. 3
Effects of perinatal intermittent hypoxia on the organization of the developing cerebellum in P12 mice. A Measurement of the thickness of the cerebellar cortex and each cerebellar layer in control (N) or hypoxic (IH) P12 mice. B Low and high magnification confocal images illustrating the thickness of the cerebellar cortex layers in control (N) or hypoxic (IH) P12 mice. The Purkinje cells were labeled by antibodies against calbindin (red) and nuclei were counterstained with DAPI (blue). C Measurement of the number of Purkinje cell bodies per frame of 413 × 103 µm3 (left) and of the volume of the calbindin-labeled Purkinje dendrites in the molecular layer of control (N) or hypoxic (IH) P12 mice, in the whole cerebellum (middle) and per cerebellar region (right). The total number of animals in each experimental group is indicated under the boxplots and represented by diamond shapes, while the transparent dots indicate individual data points. Exact p-values are indicated above the plot. EGL: external granular layer; IGL: internal granular layer; IH: intermittent hypoxia condition; N: normoxia condition; ML: molecular layer; P12: postnatal day 12; PL: Purkinje cell layer