Fig. 7

Inhibition of MAPKs attenuated D-DT-induced production of inflammatory cytokines from astrocytes. a–f ELISA determination of TNF-α (a, b), IL-1β (c, d) and IL-6 (e, f) in supernatant and lysate following astrocytes treatment with 1 µg/ml recombinant D-DT in the presence of 10 µM P38 (SB203580), 10 µM JNK (SP600125), or 10 µM ERK (PD98059) inhibitor for 24 h. g, h Western blot analysis of p65NF-κB protein levels following astrocytes treatment with 1 µg/ml recombinant D-DT in the presence of 10 µM P38 (SB203580), 10 µM JNK (SP600125), or 10 µM ERK (PD98059) inhibitor for 24 h. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)