Fig. 6

Effects of knockdown of CD74 receptor on the D-DT-mediated intracellular activation of MAPKs/NF-κB signaling in the astrocytes. a Western blot analysis of phosphorylation of ERK, P38, JNK kinase and p65NF-κB protein after siRNA2 knockdown of CD74 receptor for 48 h, prior to stimulation with 1 µg/ml recombinant D-DT protein for 12 and 24 h, respectively. b–e Quantification data as shown in a. Quantities were normalized to endogenous β-actin. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)