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Fig. 1 | Cell & Bioscience

Fig. 1

From: D-dopachrome tautomerase drives astroglial inflammation via NF-κB signaling following spinal cord injury

Fig. 1

Examination of D-DT expression changes at lesion sites following rat SCI. a Western blot analysis of D-DT following cord contusion at 0d, 1d, 4d and 7d, respectively. b Quantification data as shown in a. Quantities were normalized to endogenous β-actin. c ELISA measurement of D-DT and MIF protein levels at lesion sites following SCI at 0d, 1d, 4d and 7d, respectively. d-s Immunostaining showed colocalization of D-DT with NeuN-positive neurons (d-g) and S100β-positive astrocytes (h-k), rather than with OX42-positive microglia (l-o) or MBP-positive oligodendrocytes (p-s) before or after SCI at 4d. Rectangle indicates region magnified. Arrowheads indicate the positive signals. Scale bars, 500 μm in (d), (f), (h), (j), (l), (n), (p) and (r); 50 μm in (e), (g), (i), (k), (m), (o), (q) and (s). Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)

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