Fig. 7
Nanoparticles containing siRNA targeting QKI improved the survival rate of MRSA-infected mice. A Real-time PCR analysis of relative mRNA expression of QKI in RAW264.7 cells after treated with siQKI-LCP in various dose. B Western blots were performed to determine the effects of siQKI-LCP incubated with RAW264.7 cells 24 h before MRSA infection. Total cell lysates were subjected to western blot to analyze the protein expression of QKI, with actin as an internal control. One representative immunoblot is shown (on the left). Graphs (on the right) are representative quantification of the band intensity for immunoblots from three independent experiments. C, D C57/B6 mice were intraperitoneally injected with siQKI-LCP followed with MRSA (1 × 109) infection in the next day. c Real-time PCR analysis of relative mRNA expression of QKI in monocytes purified from peritoneal cells. d Western blots were performed to determine the effects of siQKI-LCP in purified monocytes after MRSA infection 24 h. Total cell lysates were subjected to western blot to analyze the protein expression of QKI, with actin as an internal control. One representative immunoblot is shown (on the left). Graphs (on the right) are representative quantification of the band intensity for immunoblots from three independent experiments. E, F C57/B6 mice were intraperitoneally injected with siQKI-LCP, siSC-LCP, TGX-221(3 mg/kg) respectively followed with MRSA (1 × 109) infection in the next day. (e) The survival rate of mice infected with MRSA within 3 days. (f) The body temperature of mice within 12 h was measured (n = 12/group). All the bars represented the mean of measurements from three independent experiments, and the error bars indicated ± SD. A, C are representative of three experiments, **p < 0.01, ***p < 0.001, one-way ANOVA with Tukey’s multiple comparisons test. B, D are representative of three experiments, ***p < 0.001(student’s t test). (E, F) are representative of one experiment (n = 12 mice/group). E, *p < 0.05 Log-rank (Mantel-Cox) test; F, ***p < 0.001, (student’s t test)