Skip to main content
Fig. 1 | Cell & Bioscience

Fig. 1

From: QKI degradation in macrophage by RNF6 protects mice from MRSA infection via enhancing PI3K p110β dependent autophagy

Fig. 1

Myeloid QKI deficiency is protective against MRSA infection in vivo. A Real-time PCR analysis of relative mRNA expression of QKI in PBMC of the healthy controls and in sepsis patients. B Real-time PCR analysis of relative mRNA expression of QKI in monocytes of healthy controls and in sepsis patients. (n = 6 controls and n = 10 sepsis patients). C Correlation between the PCT and mRNA expression of QKI in healthy controls and in sepsis patients. D Western blot analyzed the expression of QKI in PBMC of the healthy controls and in sepsis patients. (n = 6 controls and n = 6 sepsis patients). One representative immunoblot is shown. Graphs are representative quantification of the band intensity for immunoblots from three independent experiments (right). E Mice were injected intraperitoneally with live S. aureus (1 × 109 CFU), and the survival rate of mice infected with MRSA within 3 days. F The body temperature of mice within 12 h was measured by rectal thermometer after MRSA infection G Organs of mice after infection with MRSA 24 h were collected and homogenized. Enumeration of forming bacteria units (c.f.u) from various organs (kidney, spleen, liver) was analyzed. H Cytokines (TNF-α, IL-6, IL-1β and IL-10) level in blood were analyzed by ELISA method after infection in 24 h. I Real-time PCR analysis of relative mRNA expression of IL-6, IL-10, IL-1β and TNF-α in macrophages (F4/80+CD11b+) purified by flow cytometry from peritoneal cells after infected with MRSA 24 h. All the bars represented the mean of measurements from three independent experiments, and the error bars indicated ± SD, student’s t test. (AC) Healthy controls, n = 6; subjects with sepsis, n = 10. D, are representative of three experiments. (EF) are from one experiment (n = 12 mice/group). E, *p < 0.05 Log-rank (Mantel-Cox) test; F, * p < 0.05, (student’s t test). Symbols represent data points for each animal. (F) are from one experiment (n = 8 mice/group). (GH) are from one experiment (n = 4 mice/group). Symbols represent data points for each animal. *p < 0.05, **p < 0.01. ***p < 0.001 (student’s t test)

Back to article page