Fig. 1

Sensory neurons axonal outgrowth is promoted by osteoclasts secretome. A Representative images of dorsal root ganglia (DRG) outgrowth after treatment (stained for βIII-tubulin, scale bar 500 µm). Fresh osteoclast medium [OCm: alpha-MEM supplemented with 10% fetal bovine serum (FBS), receptor activator of nuclear factor kappa-Β ligand (RANKL) and macrophage colony-stimulating factor (M-CSF)], pre-osteoclast conditioned media (Pre-OC), and mature osteoclast conditioned media (OC) were used to stimulate embryonic DRG culture. B Automatic axonal outgrowth area quantification. Data represented as a violin plot; **p ≤ 0.01; ***p ≤ 0.001 and ****p ≤ 0.0001. C. Gene expression analysis of neurotrophic factors expressed by pre-osteoclasts (Pre-OC) and mature osteoclasts (OC), normalized for the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping gene. Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and netrins 1, 3, 4 and 5. Data represented as scatter dot plot mean ± SD