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Fig. 1 | Cell & Bioscience

Fig. 1

From: AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer

Fig. 1

SCLC cell lines with high p-AKT were sensitive to PI3K/mTOR dual inhibition. A Western blot analysis showing the expression of proteins involved in the PI3K/AKT/mTOR pathway in 13 SCLC cell lines. B Concentration-response cell survival curves of SCLC cell lines treated using GSK2126458. C Correlation between survival response (IC50) and the levels of p-AKT(T308), p-AKT(S473), PIK3CA, PTEN, p-P70S6K, and p-4EBP1. Symbols of the cell lines are the same as those denoted in B. R2, coefficient of determination. D Tumor growth curve of xenografted NCI-H446 cells in nude mice. Mice were orally (PO) administered with vehicle (10 ml/kg) or GSK2126458 (1.5 mg/kg) when the tumor size reached approximately 200 mm3 using a 5-on-2-off cycle for 2 consecutive weeks. E Western blot analysis results showing the expression of AKT, p-AKT(T308), p-AKT(S473), cleaved PARP-1 (c-PARP1), and a GAPDH loading control in xenografted NCI-H446 tumors in mice oral administered with GSK2126458 (3.0 mg/kg). Tumors were harvested at 2, 6, and 24 h after drug treatment

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Cell & Bioscience

ISSN: 2045-3701

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