Fig. 8
From: CDKN2AIP is critical for spermiogenesis and germ cell development
Reduction of Cdkn2aip impairs DNA repair, prolong cell cycle and promote apoptosis in TM4. A Cdkn2aip knockdown TM4 cell by exposure to 5 Gy γ-irradiation show more γH2AX foci after 12 h and 24 h recovery compared with control by immunofluorescence. γH2AX (red), 53BP1(green) and DAPI (blue). Scale bar, 5 μm. B Quantitative results of co-positive signal of γH2AX and 53BP1. n = 10 cells were counted. Data are presented as mean ± S.D. Student’s t test; **P < 0.01. C Western blot analysis the expression of CDKN2AIP in Cdkn2aip knockdown TM4 cell and control treated by 5 Gy γ-irradiation, GAPDH is serves as a loading control. D–H Cell cycle and apoptosis analysis of Control and Cdkn2aip knockdown TM4 cells by flow cytometry. Data are presented as mean ± S.D. Student’s t test; ***P < 0.001