Fig. 5
From: CDKN2AIP is critical for spermiogenesis and germ cell development
Disruption of the affect protamine replacement in Cdkn2aip-/- mice. A Immunofluorescence staining of histone H3 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. histone H3(green) and DAPI (blue). Scale bar, 50 μm. B Immunofluorescence staining of TNP1 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. TNP1 (green) and DAPI (blue). Scale bar, 50 μm. C and D Immunofluorescence staining of PRM1 and PRM2 in seminiferous tubules of Cdkn2aip+/+ and Cdkn2aip−/− testes. PRMs(green) and DAPI (blue). Scale bar, 50 μm. E qPCR analyses showing significantly reduced levels of Prm1 and Prm2 in 8-week-old Cdkn2aip+/+ and Cdkn2aip−/− testis. Data are presented as Data are presented as mean ± S.D. Student’s t test; *P < 0.05, ***P < 0.001. F Quantification of relative protein level of PRM1 and PRM2 by Western blotting in Cdkn2aip+/+ and Cdkn2aip−/− testis. GAPDH is serves as a loading control