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Fig. 3 | Cell & Bioscience

Fig. 3

From: CDKN2AIP is critical for spermiogenesis and germ cell development

Fig. 3

The morphology of sperm head is disrupted in Cdkn2aip−/− mice. A Light microscopy detection of sperm smear after Pap stain. Scale bar, 10 μm. B Quantification of spermatozoa with defective head from 8-week-old Cdkn2aip+/+ and Cdkn2aip−/− mice. Data are presented as mean ± S.D. Student’s t test; **P < 0.01. C Representative images of spermatozoa from 8-week-old Cdkn2aip+/+ and Cdkn2aip−/− mice stained for PNA (red) and DAPI (blue). Scale bar, 50 μm. D Abnormal sperm head was observed in Cdkn2aip+/+ and Cdkn2aip−/− mice by transmission EM. Scale bar, 2 μm. E The morphology of a wild type sperm fertilizes embryos and Cdkn2aip−/− sperm fertilizes embryos was examined by light microscopy on days 1 (D1), 2 (D2), and 3 (D3) after fertilization. Scale bar, 100 μm. F The percentage of fertilized or developing normal cells in different time periods from the wild type female oocytes after mating with Cdkn2aip+/+ and Cdkn2aip−/− mice

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