Fig. 2

Deletion Cdkn2aip results in abnormal spermatogenesis and increased male infertility. A Schematic representation of the genome editing strategy at the Cdkn2aip locus showing the sgRNAs (arrows), the corresponding coding exons (green thick lines). Sequencing results showed that 3687 bp fragment deleted. B Western blotting using the rabbit anti-CDKN2AIP antibody confirms the lack of full-length CDKN2AIP proteins in testis lysates from 8-week-old Cdkn2aip^−/− mice. GAPDH is serves as loading control. C Immunofluorescent staining of a frozen testis section from 8-week-old Cdkn2aip^+/+ and Cdkn2aip^−/− by CDKN2AIP (green) antibodies. Nuclei are labeled by DAPI (blue). Scale bar, 50 μm. D Representative images of testes from 8-week-old Cdkn2aip^−/− and Cdkn2aip^+/+ mice, and average testes/body weight ratio of 8-week-old Cdkn2aip^+/+ and Cdkn2aip^−/−, Data are presented as mean ± S.D. Student’s t test, n.s. P > 0.05. E Testicular and epididymal sections of 8-week-old Cdkn2aip^+/+ and Cdkn2aip^−/− were stained with H&E. Scale bar, 50 μm. F Litter sizes from wild type females mated with either Cdkn2aip^+/+ and Cdkn2aip^−/−. Data are presented as mean ± S.D. Student’s t test; ***P < 0.001. G Relative number of epididymal sperm number of 8-week-old Cdkn2aip^+/+ and Cdkn2aip^−/− mice (n = 6). Data are presented as mean ± S.D. Student’s t test; ***P < 0.001