Fig. 1
From: CDKN2AIP is critical for spermiogenesis and germ cell development
Expression profiles of CDKN2AIP during testicular development and spermatogenesis in mice. A Western blot analysis was performed using 20 μg protein for CDKN2AIP in various mouse tissues. GAPDH is serves as a loading control. B Isolation of nuclear protein and cytoplasmic protein from 8-week age testicular tissue by western blotting assay. Histone H3 is a nuclear-associated protein, and GAPDH is ubiquitous in the nucleus and cytoplasm. C Expression of Cdkn2aip mRNA during postnatal testicular development. Levels of Cdkn2aip mRNAs in developing testes at postnatal day 8 (P8), P10, P14, P16, P30 and P35 were analyzed using qPCR, β-actin is serves as a housekeeping gene. D Localization of CDKN2AIP in the Cdkn2aip+/+ adult testis, as detected by immunohistochemistry. CDKN2AIP is stained brown. Scale bar, 50 μm. E Immunostaining of CDKN2AIP (green) and SYCP3 (red) on chromosome spreads of spermatocytes from P35 Cdkn2aip+/+ testes, n = 3 mice for each group. Scale bars, 10 μm. F Schematic diagram showing the proposed model of CDKN2AIP expression pattern during spermatogenesis in mice