Fig. 5
From: GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis
PPP2R3A mediates TGF-β1 signaling to induce the proliferation and activation of HPF-a cells. A CCK-8 assays show that Ppp2r3a knockdown partially reversed the increase in the viability of HPF-a cells induced by TGF-β1. *p < 0.05 indicates that the cell viability of the si-Con group after TGF-β1 treatment was higher than that in the control group and that the model was therefore successfully established. #p < 0.05 indicates that the cell viability of the si-Ppp2r3a group was lower than that of the si-Con group after TGF-β1 treatment. B, C Wound healing experiments showed that the downregulation of Ppp2r3a expression attenuated cell migration induced by TGF-β1. *p < 0.05 indicates that the cell migration in the si-Con group after TGF-β1 treatment was higher than that in the control group and that the model was successfully established. #p < 0.05 indicates that the cell migration of the si-Ppp2r3a group was lower than that of the si-Con group after TGF-β1 treatment. D The combined immunofluorescence images of BrdU (green) and DAPI (blue) show that the downregulation of Ppp2r3a expression attenuated cell proliferation induced by TGF-β1. E Percentage of BrdU-positive cells in five independent experiments. *p < 0.05 indicates that the cell proliferation of the si-Con group after TGF-β1 treatment was higher than that of the control group, which showed that the model was successfully established. #p < 0.05 indicates that the cell proliferation of the si-Ppp2r3a group was lower than that of the si-Con group after TGF-β1 treatment. F, G Downregulation of Ppp2r3a expression partially reversed the increase in FN1 expression induced by TGF-β1. *p < 0.05 indicates that the expression of FN1 in the si-Con group after TGF-β1 treatment was higher than that in the control group and thus that the model was successfully established. #p < 0.05 indicates that the expression of FN1 in the si-Ppp2r3a group was lower than that in the si-Con group after TGF-β1 treatment. H Downregulation of Ppp2r3a expression had little effect on the increase in Col1 and α-SMA expression induced by TGF-β1. I, J *p < 0.05 indicates that the expression of Col1 and α-SMA in the si-Con group after TGF-β1 treatment was higher than that in the control group, which revealed that the model was successfully established