Fig. 7
From: Oxysterol derivatives Oxy186 and Oxy210 inhibit WNT signaling in non-small cell lung cancer
Oxy186 inhibits WNT pathway in xenografts. A. Gene Set Enrichment Analysis (GSEA) indicated that WNT upregulated genes, MYC upregulated genes and CCND1 upregulated genes were negatively enriched when comparing Oxy186-treated xenografts to controls. Graph shows the rank-ordered gene lists of the set. NES: normalized enrichment score. B. RT-qPCR confirmed Oxy186 inhibition of MYC and CCND1 expression, but Oxy210 treatment showed no statistical significance. Data reported as the mean (n = 6) ± SD (* p < 0.05). C–E. RT-qPCR showed that Oxy186 treatment inhibited expression of WNT target genes C, MYC-induced genes D, and CCND1-induced genes E. Data reported as the mean (n = 5) ± SD (* p < 0.05; ** p < 0.02; *** p < 0.001). F. RT-qPCR showed that Oxy186 but not Oxy210 significantly inhibited expression of EMT-related genes in xenografts. Data reported as the mean (n = 6) ± SD (* p < 0.05)