Fig.5

EGCG/RSVL suppresses Alu RNA-induced interferon expression. a–e ARPE-19 cells were transfected with Alu RNA (1 μg mL⁻¹) for 8 h, following a 3-h pretreatment with EGCG or RSVL as indicated. qPCR analysis of IFNB mRNA levels (a, c and e). Immunoblot analysis of indicated proteins, EGCG (20 μM) or RSVL (40 μM) were respectively used (b and d). f, g hTERT RPE-1 cells were transfected with Alu RNA (1 μg mL⁻¹) for 8 h, following a 3-h pretreatment with EGCG or RSVL as indicated. qPCR analysis of IFNB mRNA levels. h–k ARPE-19 cells were transfected with poly(I:C) (0.2 μg mL⁻¹) for 4 h, following a 3-h pretreatment with EGCG or RSVL as indicated. qPCR analysis of IFNB mRNA levels (h and j). Immunoblot analysis of indicated proteins, EGCG (20 μM) or RSVL (40 μM) were respectively used (i and k). Data are mean ± s.e.m. of three technical repeats (a, c, h and j) and mean ± s.e.m. of three independent experiments (e, f and g), *P < 0.05, **P < 0.01, two-tailed t test (f and g). GAPDH (b, d, i and k), loading controls. Data are representative of at least two independent experiments