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Fig.2 | Cell & Bioscience

Fig.2

From: cGAS inhibition alleviates Alu RNA-induced immune responses and cytotoxicity in retinal pigmented epithelium

Fig.2

cGAS is critical for Alu RNA-induced interferon expression. ac WT and CGAS−/− ARPE-19 cells were transfected with plasmid DNA (4 μg mL−1) for 6 h (a), genomic DNA (4 μg mL−1) for 6 h (b) or HT-DNA (2 μg mL−1)for 6 h (c) and IFNB mRNA levels were analyzed by qPCR. d Immunoblot analysis of indicated proteins in WT and CGAS−/− ARPE-19 cells that transfected with HT-DNA. e WT and CGAS−/− ARPE-19 cells were transfected with Alu RNA (1 μg mL−1) for 8 h and IFNB mRNA levels was analyzed by qPCR. f Immunoblot analysis of indicated proteins in WT and CGAS−/− ARPE-19 cells that transfected with Alu RNA. g, h WT and CGAS−/− U937 cells were transfected with HT-DNA (2 μg mL−1) (g) or Alu RNA (1 μg mL−1) (h) for 3 h and IFNB mRNA levels was analyzed by qPCR. Data are mean ± s.e.m. of three technical repeats (a, b, c, e, g and h). GAPDH (d and f), loading controls. Data are representative of at least two independent experiments

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