Fig.2

cGAS is critical for Alu RNA-induced interferon expression. a–c WT and CGAS^−/− ARPE-19 cells were transfected with plasmid DNA (4 μg mL⁻¹) for 6 h (a), genomic DNA (4 μg mL⁻¹) for 6 h (b) or HT-DNA (2 μg mL⁻¹)for 6 h (c) and IFNB mRNA levels were analyzed by qPCR. d Immunoblot analysis of indicated proteins in WT and CGAS^−/− ARPE-19 cells that transfected with HT-DNA. e WT and CGAS^−/− ARPE-19 cells were transfected with Alu RNA (1 μg mL⁻¹) for 8 h and IFNB mRNA levels was analyzed by qPCR. f Immunoblot analysis of indicated proteins in WT and CGAS^−/− ARPE-19 cells that transfected with Alu RNA. g, h WT and CGAS^−/− U937 cells were transfected with HT-DNA (2 μg mL⁻¹) (g) or Alu RNA (1 μg mL⁻¹) (h) for 3 h and IFNB mRNA levels was analyzed by qPCR. Data are mean ± s.e.m. of three technical repeats (a, b, c, e, g and h). GAPDH (d and f), loading controls. Data are representative of at least two independent experiments