Fig.1

dsRNA induced the interferon production in ARPE-19 cells. a, b ARPE-19 cells were transfected with Alu RNA (0.5 μg mL⁻¹ or 1 μg mL⁻¹) for 8 h. IFNB mRNA levels was analyzed by qPCR (a) and immunoblot analysis of indicated proteins (b). c, d ARPE-19 cells were transfected with Alu RNA (1 μg mL⁻¹) for indicated hours and IFNB mRNA levels was analyzed by qPCR (c), immunoblot analysis of indicated proteins (d). e ARPE-19 cells were transfected with Alu RNA (1 μg mL⁻¹) for 24 h and the secreted IFN-β was measured by ELISA. f, g ARPE-19 cells were transfected with poly(I:C) as indicated concentrations for 4 h and IFNB mRNA levels was analyzed by qPCR (f), and the expression of indicated proteins were analyzed with immunoblotting (g). h, i ARPE-19 cells were transfected with poly(I:C) (0.2 μg mL⁻¹) as indicated and IFNB mRNA levels was analyzed by qPCR (h). The expression of indicated proteins were analyzed with immunoblotting (i). j ARPE-19 cells were transfected with poly(I:C) (1 μg mL⁻¹) for 24 h and the secreted IFN-β was measured by ELISA. Data are mean ± s.e.m. of three independent experiments (e and j) and mean ± s.e.m. of three technical repeats (a, c, f and h). ND, not detected (e and j). GAPDH (b, d, g and i), loading controls. Data are representative of at least two independent experiments