Fig. 5
Inhibition of AgRPARC neurons in fasted animals reduces CRF content in the PVN and increases its accumulation in the median eminence: Representative photomicrographs (D and E) and quantitative CRF-IR (green) cell number (A) and mean intensity (B and C) in both PVN and median eminence of Agrp-IRES-Cre animals that received intra ARC injection of AAV-DIO-mCherry or AAV-DIO-hM4D(Gi)-mCherry (red) submitted to 36 h of fasting and treated 5 i.p. injections CNO (1 mg/kg). Tissue samples obtained from experimental procedure described in the Fig. 3 were used for CRF immunostaining studies. The cell nuclei were staining with Hoechst (blue). Data are expressed as mean ± SEM and were analyzed by unpaired t test: *p < 0.05 vs Agrp-IRES-Cre:: AAV-DIO-mCherry fasted and treated with CNO