Fig. 4From: Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fastingChemogenetic inhibition of AgRPARC neurons reduces PVN neuronal activity without affecting plasma corticosterone levels in fasted animals: Number of mCherry positive neurons (A), FosB (left y scale and color bars) and double mCherry/FosB (right y scale and black bars) expression in the ARC (B), representative photomicrographs (×10 and insert ×20) of double immunostaining mCherry/FosB in the ARC (C), body weight change (D), plasma leptin (E), glucose (F) and corticosterone (G) levels, PVN FosB expressing neurons (H) and representative photomicrographs (×10 and insert ×40) of FosB in the PVN (I) of adult (8–12 weeks) male Agrp-IRES-Cre mice (n = 14–18) that received intra ARC injection of AAV-DIO-mCherry or AAV-DIO-hM4D(Gi)-mCherry submitted to 36 h of fasting and treated with 5 i.p. injections CNO (1 mg/kg) or saline. The i.p. injections were performed each 8 h and the animals were euthanized at 10am as described in the Additional file 1: Fig. S1. Data are expressed as mean ± SEM and were analyzed by two-way ANOVA followed by post hoc Tukey’s multiple comparison (D and G) or unpaired t test or Mann–Whitney (A, B, E, F and H). *p < 0.05 vs saline; #p < 0.05 vs Agrp-IRES-Cre::AAV-DIO-mCherryBack to article page