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Fig. 3 | Cell & Bioscience

Fig. 3

From: Myometrial oxidative stress drives MED12 mutations in leiomyoma

Fig. 3

Evaluation of oxidized guanine in primary myometrial cells with ROS exposure in vitro, and ROS-induced gene expression in myometrial cells by RNA-seq analysis. a DHE staining (ROS) in 3D spheroid cultures of myometrial cells treated with control and PQ. b and c Immunohistochemistry (b) and quantitative H-scores (c) for 8-OHdG and γH2AX in 3D cultured myometrial cells treated with control and PQ. d Quantitative analysis of the absence of base site (apurinic/apyrimidinic (AP)) in genomic DNA treated for 6 hrs (blue bars) and 18 hrs (red bars) in myometrial cells treated with control and PQ . e Heatmaps (n=4, NUP245, NUP246, NUP237, NUP240) showed 325 genes treated with PQ (178 upregulated and 147 downregulated). f Volcano plot illustrated significantly up (Red) and down (blue) regulated genes. g Gene enrichment analysis of six major pathways upregulate by PQ treatment. *p<0.05; **p<0.01

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