Fig. 2

Oxidized DNA, DNA damage response, and ROS response in myometrium with or without LM. a Diagram illustrated tissue microarray (TMA) preparation in myometrium without LM (MM^-LM) and with LM (MM^+LM). b Immunohistochemistry for 8-OHdG in MM^-LM and MM^+LM. c Histobars showed semi-quantitative immunoscores for 8-OHdG in MM^-LM and MM^+LM (n=10 for each). d Percentage of cells with ≥3 nuclear dots of immunofluorescent stain for γH2AX in MM^-LM and MM^+LM (n=10 for each). e Heatmap showed the expression of 3446 genes were significantly different between MM^-LM and MM^+LM (n=3 for each). (MM^+LM vs. MM^-LM: 1,596 upregulated and 1,850 downregulated). f Volcano plot compared a global transcriptional change across the groups (MM^-LM vs. MM^+LM, n=3). Genes involving DNA repair (yellow), P53 (blue), ROS (red), AKT (green) and hypoxia (purple) pathways were highlighted. g GO pathway analysis revealed the top-ranking pathways different in MM^+LM in comparison to MM^-LM (p value ranked from low in red to high in blue). h Pathway analysis from GSEA revealed significantly changed pathways: ROS, hypoxia, oxidative phosphorylation and DNA repair pathways. *p<0.05; **p<0.01