Fig. 5From: SENP6 induces microglial polarization and neuroinflammation through de-SUMOylation of Annexin-A1 after cerebral ischaemia–reperfusion injurySENP6 overexpression in microglia promotes neuronal apoptosis under coculture conditions. A Primary cultured microglia were infected with adenovirus encoding the SENP6 coding sequence or shRNA sequence for 48 h and were cocultured with neurons, followed by OGD/R treatment. B Representative TUNEL staining labelling of apoptotic neurons. C Quantification of apoptotic neurons in B. D Collecting the supernatant in neuronal coculture. LDH assay indicating LDH release. E CCK-8 assay detecting neuronal cell viability when cocultured with adenovirus encoding SENP6 or sh. SENP6-infected primary microglia. F Immunoblots indicating the protein expression of representative apoptosis molecules in cocultured primary neurons. G–I Quantitative analysis of cleaved caspase-3, cleaved caspase-9, and cleaved PARP protein expression in F. Scale bar = 20 μm. Data are presented as the mean ± S.E.M. from at least three dependent experiments and analysed by one-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001Back to article page