Fig. 5

CRLM1 interacts with hnRNPK and promotes its nuclear localization. A CRLM1–protein interaction network. Histone, RNA binding proteins (RBP), transcriptional factors (TF) and other proteins are grouped separately. Hexagon indicates the metastasis-related genes from HCMDB database. The protein association networks were constructed using STRING. B Western validation of HNRNPK protein retrieved by CRLM1. C hnRNPK RIP-qPCR. D Predicted second structure of CRLM1 which contains hnRNPK binding motif at 403 to 410. E Localization of CRLM1 and hnRNPK in HCT116-NC and HCT116-CRLM1 cells by fluorescence in situ hybridization and immunofluorescence. Blue represents DAPI staining of the nucleus, green represents GFP staining of the cytoplasm, red represents Cy3-labeled probe against CRLM1 RNA and yellow represents Aluor 647 against hnRNPK-antibody. F The hnRNPK protein from purified nuclear fractions and cytosolic fraction in HCT116 and SW620 cells transfected with CRLM1-OE was examined using western blotting