Fig. 3

Visualization of bioinformatics analysis results. a Heatmap plots showing the expression of MSC surface markers. The bar chart above each heatmap represents the different cell subsets with different colors. Moreover, the UMAP plots show the distributions of different cell subsets in low-dimensional space. Red dots represent MSCs, and green dots represent unknown cells. b Violin plots showing the distribution of the expression of marker genes among different MSC subsets with different colors. c Dendrogram plot showing the similarity of MSC subsets. Dots with different colors correspond to different MSC subsets. The expression patterns of MSC subsets are closer when the dots are closer together in the dendrogram plot. d PCA plots showing the distribution of FSMSCs and HuMSCs in low-dimensional space before and after integration. Red dots represent FSMSCs, and green dots represent HuMSCs. The left plot shows the batch effect of the scRNA-seq data before integration. The right plot shows the batch effect of the scRNA-seq data after integration. e TSNE plots showing the distribution of different MSC subsets in low-dimensional space before and after annotation. f The left bar plots show the proportions of different MSC subsets between FSMSCs and HuMSCs. Different colors represent different MSCs subsets. The right bar plots show the proportions of different cell cycle phases among different MSC subsets. Different colors represent different cell cycle phases. g Circle plot showing the expression of the immune-related differentially expressed genes in immune MSCs. The color intensity of the grid represents the degree of gene expression. A bluer color indicates lower gene expression