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Fig. 6 | Cell & Bioscience

Fig. 6

From: Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1/ATG14 pathway

Fig. 6

The decreased invasion and migration caused by Siglec-15 silencing could be reversed by Beclin-1 overexpression. a Beclin-1 overexpression increased MMP-9 levels and induced EMT, while Siglec-15 expression did not change (left). Overexpression of Beclin-1 promoted the decreased EMT and autophagy processes caused by Siglec-15 silencing, and the expression levels of related protein markers were tested by Western blotting (right). b Immunofluorescence assays of the cytoskeleton were used to detect changes in the cytoskeleton after Beclin-1 overexpression combined with Siglec-15 silencing. c An immunofluorescence assay of LC3 was used to verify changes in autophagy after Beclin-1 overexpression combined with Siglec-15 silencing. d Transwell assays indicate that the decreased invasion and migration caused by Siglec-15 silencing could be reversed by Beclin-1 overexpression. Data are presented as the mean ± SD from three experiments. **P < 0.01, ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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