Fig. 5From: Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1/ATG14 pathwayAutophagy inhibition attenuates the migration and invasion of osteosarcoma cells by inactivating epithelial–mesenchymal transition and affecting cytoskeletal rearrangement. a SiRNA sequences targeting Beclin-1 were used, and the knockdown efficiency was detected using Western blotting. Beclin-1 silencing inhibits the epithelial–mesenchymal transition and MMP-9 expression via Western blot. b Inhibition of autophagy by Beclin-1 knockdown significantly restrained the migration and invasion of osteosarcoma cells. c In contrast to the Beclin-1-depletion group, the lamellipodial prominences and F-actin filaments gathered near the edge of the control group. Representative images are shown. Cell nuclei are stained with DAPI. The scale bar represents 50 μm. d Beclin-1 silencing results in decreased RhoA activationBack to article page