Fig. 3

Siglec-15 knockdown suppresses the migration and invasion of osteosarcoma cells in vitro. a ShRNA sequences targeting Siglec-15 were used, and the knockdown efficiency was measured by Western blotting. Siglec-15 depletion inhibits epithelial–mesenchymal transition, as shown by Western blotting. b Transwell assays and wound healing assays indicate that Siglec-15 depletion significantly decreased the migration and invasive capacity of KHOS and U2OS cells. c Cytoskeletal assays show that more conspicuous lamellipodial protrusions were formed in the control cells than in the Siglec-15-depletion group. Representative images are shown. Cell nuclei are stained with DAPI. The scale bar represents 50 μm. Decreased RhoA activation was also observed in the Siglec-15-depletion group. All experiments were repeated three times. Data are presented as the mean ± SD. **P < 0.01, ***P < 0.001