Fig. 7
From: Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis
NETs induce thrombogenicity in HBMECs. HBMECs were incubated with isolated NETs in the presence or absence of DNase I, APC and sivelestat. a, b Representative images showing ICAM-1 (red) and VCAM-1 (green) in treated ECs as observed by confocal microscopy. c The expression of ICAM-1 (red) and VCAM-1 (green) was measured by western blotting. d, e Representative images showing TF (red) and CD31 (green) expression on ECs. f The expression of TF on ECs was measured by western blotting. g, h Representative images showing VWF (red) and CD31 (green) expression on ECs incubated with NETs. i The expression of VWF in the supernatant in each group. (j) Fibrin formation in ECs incubated with NETs (0.5 μg/mL) in the presence of DNase I, APC and sivelestat. Statistics: Ordinary one-way ANOVA (i, j). The inset scale bars in a, b, d, e, g and h are 20 μm. ***P < 0.001.****P < 0.0001