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Fig. 7 | Cell & Bioscience

Fig. 7

From: N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer

Fig. 7

CD44 silencing inhibited tumorigenesis of PC, in agreement with the changes observed after CELF2 overexpression. A, B The effects of CD44 on pancreatic cancer cells were analyzed by transfecting BxPC-3 and MIA PaCa2 cells with different siRNAs (siCD44#1, siCD44#2, and siCD44#3). C Western blot was used to analyze the protein level of CD44 after the knockdown of CD44 in BxPC-3 and MIA Paca2 cell. D, E CCK-8 assay. F Plate clone formation assay. G Transwell assay. H EMT markers in CD44 knockdown cells were detected by western blot. Data are shown as the mean ± SD of three replicates. *P < 0.05, **P < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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