Fig. 4From: N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancerCELF2 downregulation inhibits apoptosis and maintains the stemness of PC cells. A–C Representative images of sphere formation induced by CELF2 overexpression in BxPC-3 and MIA PaCa2 cells. The surviving colonies were measured for the number of tumorspheres. D The expression levels of PC cell stemness markers, including CD133, OCT4, NANOG, and SOX-2, were examined by western blot. E Flow cytometry was used to assess apoptosis of PC cells in the CELF2 overexpression and NC groups. F The expression levels of apoptosis markers, including BAX, BCL-2, and caspase-3, were examined by western blot. Data are shown as the mean ± SD of three replicates. *P < 0.05, **P < 0.01Back to article page