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Fig. 4 | Cell & Bioscience

Fig. 4

From: N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer

Fig. 4

CELF2 downregulation inhibits apoptosis and maintains the stemness of PC cells. A–C Representative images of sphere formation induced by CELF2 overexpression in BxPC-3 and MIA PaCa2 cells. The surviving colonies were measured for the number of tumorspheres. D The expression levels of PC cell stemness markers, including CD133, OCT4, NANOG, and SOX-2, were examined by western blot. E Flow cytometry was used to assess apoptosis of PC cells in the CELF2 overexpression and NC groups. F The expression levels of apoptosis markers, including BAX, BCL-2, and caspase-3, were examined by western blot. Data are shown as the mean ± SD of three replicates. *P < 0.05, **P < 0.01

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