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Fig. 3 | Cell & Bioscience

Fig. 3

From: N6-methyladenosine-mediated CELF2 regulates CD44 alternative splicing affecting tumorigenesis via ERAD pathway in pancreatic cancer

Fig. 3

CELF2 upregulation inhibited the proliferation, migration, and invasion of PC cells. A and B Western blot (A) and qRT-PCR (B) analyses were used to measure the protein and mRNA levels of CELF2 in various PC cell lines and normal pancreatic cells. C Western blot was used to measure the protein levels of CELF2 in BxPC-3 and MIA PaCa2 cells 48 h after transfection with a CELF2 overexpression plasmid. D and E The CCK-8 assay revealed CELF2 overexpression inhibited the proliferation of BxPC-3 (D) and MIA PaCa2 cells (E). F CELF2 overexpression inhibited colony formation of BxPC-3 and MIA PaCa2 cells in vitro. G Transwell analysis revealed that the invasion and migration of BxPC-3 and MIA PaCa2 cells were significantly decreased when CELF2 was overexpressed. H EMT markers in cells with high expression levels of CELF2 were detected by western blot. Data are shown as the mean ± SD of three replicates. *P < 0.05, **P < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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