Fig. 5
From: Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis
SaCas9 and KKH SaCas9 mediated genome editing are efficient enough for G0 phenotyping in X. tropicalis. a Wild-type stage 44 tadpoles. b Stage 44 tadpoles show minor perturbation of cutaneous and ocular pigmentation upon SaCas9-mediated disruption of tyr. c, d Stage 44 tadpoles show cardiac malformation and edema caused by SaCas9-mediated disruption of tbx5. e, f Stage 44 tadpoles show albinism caused by KKH SaCas9-mediated disruption of tyr. g, h Stage 44 tadpoles show cardiac malformation and edema caused by KKH SaCas9-mediated disruption of tbx5. For b–h, the penetrance of each phenotype is well in line with the corresponding genome editing efficiency. For all panels, the phenotype frequency is given in the representative image. Scale bars, 2 mm. For target site designation, the gene name is in the middle, the prefixes Sa- and KKH- represent SaCas9 and KKH SaCas9, respectively. The suffixes -T1 and -T2 represent the first and the second target site designed in a given gene, respectively