Fig. 3

VQR Cas9, SpG Cas9, SpRY Cas9 and xCas9 3.7 have limited genome editing efficacy in X. tropicalis. a VQR Cas9, SpG Cas9, and SpRY Cas9 induced indel frequency in X. tropicalis embryos, as detected by deep-sequencing. b–d Some of the deep-sequencing data show the mutations induced by VQR Cas9, SpG Cas9, and SpRY Cas9. The wild-type sequence is shown at the top with the target site highlighted in yellow and the PAM sequence in blue text. Red dashes indicate deletions and lowercase letters in red indicate insertions or mutations. For all panels, the numbers in parentheses show the percentage of this sequence in the total sequence reads. e Summary of the xCas9 3.7 targeted genome-editing efficiency based on gray value analyses of the T7EI data. f DNA sequences show xCas9 3.7 induced mutations, as detected by Sanger DNA sequencing. For each panel, the wild-type sequence is shown at the top with the target site highlighted in yellow and the PAM sequence in blue text. Red dashes indicate deletions and lowercase letters in red indicate insertions or mutations. The numbers in parentheses represent the ratio of this sequence in the total colonies sequenced. For target site designation, in addition to the gene name, the name of the Cas9 variant is used as a prefix and in some cases the PAM motif is indicated as a suffix