Table 1 List of primers used for RT-PCR analysis and of siRNAs for silencing experiments
Gene | Sequence |
|---|---|
β-actin | 5ʹ-AGCCATGTACGTAGCCATCC-3ʹ 5ʹ-CTCTCAGCTGTGGTGGTGAA-3ʹ |
IL-1β | 5ʹ-ACTCATTGTGGCTGTGGAGA-3ʹ 5ʹ-TAGCAGGTCGTCATCATCCC-3ʹ |
IL-6 | 5ʹ-TGATGGATGCTTCCAAACTG-3ʹ 5ʹ-GAGCATTGGAAGTTGGGGTA-3ʹ |
IL-8 | 5ʹ-GAAGATAGATTGCACCGA-3ʹ 5ʹ-CATAGCCTCTCACACATTTC-3ʹ |
MCP-1 | 5ʹ-GCCAGTGAATGAGTAGCAAG-3ʹ 5ʹ-CTTCTGGGCCTGTTGTTCAC-3ʹ |
TNF-α | 5ʹACTGAACTTCGGGGTGATTG-3ʹ 5ʹGCTTGGTGGTTTGCTACGAC-5ʹ |
CamKIIα siRNA#1 (27mer duplex) | rArCrArArGrArArGrArArUrGrArUrGrGrCrGrUrGrArArGrGrA |
CamKIIα siRNA#2 (27mer duplex) | rGrGrCrCrUrGrGrArCrUrUrUrCrArUrCrGrArUrUrCrUrArTrT |