Fig. 9

DNMT1 inhibitor 5-Aza protected from renal injuries in vivo. A Scr and Upro levels of the C57 + PBS(NC), C57 + 5-Aza (NC + 5-Aza, 1 mg/kg weight), HBV + PBS, HBV + 5-Aza (1 mg/kg weight) groups at 24 weeks were measured. B Representative Masson’s stained kidney sections of different groups. Scale bar: 25 μm. C Immunohistochemistry for DNMT1, IL-6, and TNF-α in the renal cortex from experimental mice. Scale bar: 100 μm. D Western blotting to evaluate HBx and DNMT1 expression in the kidneys from the experimental mice. E Western blotting to evaluate PTEN, VDR, and SNAI1 expression in the kidneys from the experimental mice. F Western blotting on IL-6, TNF-α, P65, p-P65, IκBα and p-IκBα levels in the kidneys from the experimental mice. G Western blotting to evaluate Akt, p-Akt, mTOR, and p-mTOR levels in the experimental mouse kidneys. Results are expressed as mean ± SD of 3 separate assays. *P < 0.05; **P < 0.01